{"id":12591,"date":"2025-12-21T03:40:26","date_gmt":"2025-12-21T01:40:26","guid":{"rendered":"https:\/\/stemlix.com\/?p=12591"},"modified":"2025-12-21T03:40:26","modified_gmt":"2025-12-21T01:40:26","slug":"hematopoietic-stem-cell-markers","status":"publish","type":"post","link":"https:\/\/stemlix.com\/en\/hematopoietic-stem-cell-markers\/","title":{"rendered":"hematopoietic stem cell markers"},"content":{"rendered":"<div class=\"markdown prose dark:prose-invert w-full break-words light markdown-new-styling\"><p data-start=\"0\" data-end=\"377\">Hematopoietic stem cells (HSCs) are the progenitors of all blood cell types, including red blood cells, white blood cells, and platelets. These cells are typically found in the bone marrow and are essential for maintaining the body\u2019s blood cell population throughout life. Identifying and isolating HSCs is crucial for research in regenerative medicine and stem cell therapies.<\/p>\n<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_82_2 counter-hierarchy ez-toc-counter ez-toc-grey ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title\" style=\"cursor:inherit\">\u0645\u062d\u062a\u0648\u064a\u0627\u062a \u0627\u0644\u0635\u0641\u062d\u0629<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #999;color:#999\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewbox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #999;color:#999\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewbox=\"0 0 24 24\" version=\"1.2\" baseprofile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1' ><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/stemlix.com\/en\/hematopoietic-stem-cell-markers\/#Key_Hematopoietic_Stem_Cell_Markers\" >Key Hematopoietic Stem Cell Markers<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/stemlix.com\/en\/hematopoietic-stem-cell-markers\/#Combinations_of_Markers_for_HSC_Identification\" >Combinations of Markers for HSC Identification<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/stemlix.com\/en\/hematopoietic-stem-cell-markers\/#Importance_of_Hematopoietic_Stem_Cell_Markers\" >Importance of Hematopoietic Stem Cell Markers<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/stemlix.com\/en\/hematopoietic-stem-cell-markers\/#Frequently_Asked_Questions_FAQs\" >Frequently Asked Questions (FAQs)<\/a><\/li><\/ul><\/nav><\/div>\n<h3 data-start=\"379\" data-end=\"418\"><span class=\"ez-toc-section\" id=\"Key_Hematopoietic_Stem_Cell_Markers\"><\/span>Key Hematopoietic Stem Cell Markers<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<ol data-start=\"420\" data-end=\"3473\">\n<li data-start=\"420\" data-end=\"875\">\n<p data-start=\"423\" data-end=\"433\"><strong data-start=\"423\" data-end=\"431\">CD34<\/strong><\/p>\n<ul data-start=\"437\" data-end=\"875\">\n<li data-start=\"437\" data-end=\"643\">\n<p data-start=\"439\" data-end=\"643\"><strong data-start=\"439\" data-end=\"454\">Description<\/strong>: CD34 is a cell surface glycoprotein that is widely used as a marker for identifying HSCs. It is considered one of the most prominent markers in the isolation and characterization of HSCs.<\/p>\n<\/li>\n<li data-start=\"647\" data-end=\"875\">\n<p data-start=\"649\" data-end=\"875\"><strong data-start=\"649\" data-end=\"657\">Role<\/strong>: CD34 marks a population of progenitor cells that are capable of differentiation into various blood cell types. However, it is not exclusive to stem cells and can also be found on other hematopoietic progenitor cells.<\/p>\n<\/li>\n<\/ul>\n<\/li>\n<li data-start=\"877\" data-end=\"1244\">\n<p data-start=\"880\" data-end=\"890\"><strong data-start=\"880\" data-end=\"888\">CD38<\/strong><\/p>\n<ul data-start=\"894\" data-end=\"1244\">\n<li data-start=\"894\" data-end=\"1010\">\n<p data-start=\"896\" data-end=\"1010\"><strong data-start=\"896\" data-end=\"911\">Description<\/strong>: CD38 is another cell surface marker that is often used in combination with CD34 to identify HSCs.<\/p>\n<\/li>\n<li data-start=\"1014\" data-end=\"1244\">\n<p data-start=\"1016\" data-end=\"1244\"><strong data-start=\"1016\" data-end=\"1024\">Role<\/strong>: HSCs are typically CD34+ and CD38-. This phenotype helps to distinguish true stem cells from more differentiated progenitor cells. CD38 is a marker for more mature cells and is generally absent on primitive stem cells.<\/p>\n<\/li>\n<\/ul>\n<\/li>\n<li data-start=\"1246\" data-end=\"1647\">\n<p data-start=\"1249\" data-end=\"1278\"><strong data-start=\"1249\" data-end=\"1276\">Lin- (Lineage-negative)<\/strong><\/p>\n<ul data-start=\"1282\" data-end=\"1647\">\n<li data-start=\"1282\" data-end=\"1439\">\n<p data-start=\"1284\" data-end=\"1439\"><strong data-start=\"1284\" data-end=\"1299\">Description<\/strong>: &#8220;Lin-&#8221; refers to cells that do not express markers for differentiated blood cell types. A lineage-negative population is enriched in HSCs.<\/p>\n<\/li>\n<li data-start=\"1443\" data-end=\"1647\">\n<p data-start=\"1445\" data-end=\"1647\"><strong data-start=\"1445\" data-end=\"1453\">Role<\/strong>: HSCs are usually Lin-, meaning they do not express surface markers of mature blood cells (like CD3, CD14, CD19, etc.), which makes this marker useful for isolating undifferentiated stem cells.<\/p>\n<\/li>\n<\/ul>\n<\/li>\n<li data-start=\"1649\" data-end=\"2035\">\n<p data-start=\"1652\" data-end=\"1685\"><strong data-start=\"1652\" data-end=\"1683\">Sca-1 (Stem Cell Antigen-1)<\/strong><\/p>\n<ul data-start=\"1689\" data-end=\"2035\">\n<li data-start=\"1689\" data-end=\"1848\">\n<p data-start=\"1691\" data-end=\"1848\"><strong data-start=\"1691\" data-end=\"1706\">Description<\/strong>: Sca-1 is a glycoprotein expressed on murine HSCs, commonly used in combination with other markers to identify stem cell populations in mice.<\/p>\n<\/li>\n<li data-start=\"1852\" data-end=\"2035\">\n<p data-start=\"1854\" data-end=\"2035\"><strong data-start=\"1854\" data-end=\"1862\">Role<\/strong>: In combination with CD34 and Lin-, Sca-1 helps to identify a rare subset of HSCs. It is a marker for stem cells and progenitors that can give rise to all blood cell types.<\/p>\n<\/li>\n<\/ul>\n<\/li>\n<li data-start=\"2037\" data-end=\"2378\">\n<p data-start=\"2040\" data-end=\"2058\"><strong data-start=\"2040\" data-end=\"2056\">CD90 (Thy-1)<\/strong><\/p>\n<ul data-start=\"2062\" data-end=\"2378\">\n<li data-start=\"2062\" data-end=\"2178\">\n<p data-start=\"2064\" data-end=\"2178\"><strong data-start=\"2064\" data-end=\"2079\">Description<\/strong>: CD90, also known as Thy-1, is another marker used to isolate HSCs, particularly in human studies.<\/p>\n<\/li>\n<li data-start=\"2182\" data-end=\"2378\">\n<p data-start=\"2184\" data-end=\"2378\"><strong data-start=\"2184\" data-end=\"2192\">Role<\/strong>: CD90 is often co-expressed with other markers like CD34 in more primitive HSCs. Its presence helps identify more undifferentiated and primitive populations of hematopoietic stem cells.<\/p>\n<\/li>\n<\/ul>\n<\/li>\n<li data-start=\"2380\" data-end=\"2737\">\n<p data-start=\"2383\" data-end=\"2393\"><strong data-start=\"2383\" data-end=\"2391\">CD45<\/strong><\/p>\n<ul data-start=\"2397\" data-end=\"2737\">\n<li data-start=\"2397\" data-end=\"2530\">\n<p data-start=\"2399\" data-end=\"2530\"><strong data-start=\"2399\" data-end=\"2414\">Description<\/strong>: CD45 is a common leukocyte antigen expressed on all leukocytes, including hematopoietic stem and progenitor cells.<\/p>\n<\/li>\n<li data-start=\"2534\" data-end=\"2737\">\n<p data-start=\"2536\" data-end=\"2737\"><strong data-start=\"2536\" data-end=\"2544\">Role<\/strong>: While CD45 expression is generally low on HSCs, it helps in distinguishing hematopoietic cells from other types of stem cells, especially in studies involving cell sorting and identification.<\/p>\n<\/li>\n<\/ul>\n<\/li>\n<li data-start=\"2739\" data-end=\"3112\">\n<p data-start=\"2742\" data-end=\"2766\"><strong data-start=\"2742\" data-end=\"2764\">CD133 (Prominin-1)<\/strong><\/p>\n<ul data-start=\"2770\" data-end=\"3112\">\n<li data-start=\"2770\" data-end=\"2895\">\n<p data-start=\"2772\" data-end=\"2895\"><strong data-start=\"2772\" data-end=\"2787\">Description<\/strong>: CD133 is a marker that is highly expressed on hematopoietic stem cells, particularly in human populations.<\/p>\n<\/li>\n<li data-start=\"2899\" data-end=\"3112\">\n<p data-start=\"2901\" data-end=\"3112\"><strong data-start=\"2901\" data-end=\"2909\">Role<\/strong>: CD133+ cells are enriched for HSCs and have been linked to self-renewal and multi-lineage differentiation. It is considered a key marker for isolating and characterizing human hematopoietic stem cells.<\/p>\n<\/li>\n<\/ul>\n<\/li>\n<li data-start=\"3114\" data-end=\"3473\">\n<p data-start=\"3117\" data-end=\"3134\"><strong data-start=\"3117\" data-end=\"3132\">KIT (CD117)<\/strong><\/p>\n<ul data-start=\"3138\" data-end=\"3473\">\n<li data-start=\"3138\" data-end=\"3242\">\n<p data-start=\"3140\" data-end=\"3242\"><strong data-start=\"3140\" data-end=\"3155\">Description<\/strong>: CD117 is a receptor for stem cell factor (SCF), and its expression is common in HSCs.<\/p>\n<\/li>\n<li data-start=\"3246\" data-end=\"3473\">\n<p data-start=\"3248\" data-end=\"3473\"><strong data-start=\"3248\" data-end=\"3256\">Role<\/strong>: HSCs that are positive for CD117 (KIT+) are capable of proliferation and differentiation. This marker is also used to identify a broader population of hematopoietic progenitors and is important in stem cell biology.<\/p>\n<\/li>\n<\/ul>\n<\/li>\n<\/ol>\n<h3 data-start=\"3475\" data-end=\"3525\"><span class=\"ez-toc-section\" id=\"Combinations_of_Markers_for_HSC_Identification\"><\/span>Combinations of Markers for HSC Identification<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p data-start=\"3527\" data-end=\"3645\">To reliably isolate and identify hematopoietic stem cells, combinations of these markers are often used. For instance:<\/p>\n<ul data-start=\"3647\" data-end=\"3889\">\n<li data-start=\"3647\" data-end=\"3764\">\n<p data-start=\"3649\" data-end=\"3764\"><strong data-start=\"3649\" data-end=\"3669\">CD34+ CD38- Lin-<\/strong>: This combination is one of the most commonly used to identify a population enriched for HSCs.<\/p>\n<\/li>\n<li data-start=\"3765\" data-end=\"3889\">\n<p data-start=\"3767\" data-end=\"3889\"><strong data-start=\"3767\" data-end=\"3783\">CD133+ CD34+<\/strong>: These markers are often used to enrich for stem and progenitor cells in human hematopoietic populations.<\/p>\n<\/li>\n<\/ul>\n<h3 data-start=\"3891\" data-end=\"3940\"><span class=\"ez-toc-section\" id=\"Importance_of_Hematopoietic_Stem_Cell_Markers\"><\/span>Importance of Hematopoietic Stem Cell Markers<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p data-start=\"3942\" data-end=\"3997\">Identifying HSCs using specific markers is crucial for:<\/p>\n<ul data-start=\"3998\" data-end=\"4384\">\n<li data-start=\"3998\" data-end=\"4108\">\n<p data-start=\"4000\" data-end=\"4108\"><strong data-start=\"4000\" data-end=\"4031\">Bone Marrow Transplantation<\/strong>: Ensuring that the transplanted cells can regenerate a healthy blood system.<\/p>\n<\/li>\n<li data-start=\"4109\" data-end=\"4245\">\n<p data-start=\"4111\" data-end=\"4245\"><strong data-start=\"4111\" data-end=\"4127\">Gene Therapy<\/strong>: Targeting HSCs for gene editing techniques that may offer cures for genetic blood disorders like sickle cell anemia.<\/p>\n<\/li>\n<li data-start=\"4246\" data-end=\"4384\">\n<p data-start=\"4248\" data-end=\"4384\"><strong data-start=\"4248\" data-end=\"4273\">Regenerative Medicine<\/strong>: Understanding how to regenerate or repair blood cells in diseases affecting the bone marrow or immune system.<\/p>\n<\/li>\n<\/ul>\n<p data-start=\"4386\" data-end=\"4632\">Understanding these markers enables researchers and clinicians to better understand the biology of hematopoietic stem cells and improve treatments related to hematopoiesis, such as bone marrow failure, leukemia, and other hematological disorders.<\/p>\n<h3 data-start=\"4634\" data-end=\"4671\"><span class=\"ez-toc-section\" id=\"Frequently_Asked_Questions_FAQs\"><\/span>Frequently Asked Questions (FAQs)<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<ol data-start=\"4673\" data-end=\"5979\" data-is-only-node=\"\" data-is-last-node=\"\">\n<li data-start=\"4673\" data-end=\"4945\">\n<p data-start=\"4676\" data-end=\"4740\"><strong data-start=\"4676\" data-end=\"4740\">What is the most common marker for hematopoietic stem cells?<\/strong><\/p>\n<ul data-start=\"4744\" data-end=\"4945\">\n<li data-start=\"4744\" data-end=\"4945\">\n<p data-start=\"4746\" data-end=\"4945\"><strong data-start=\"4746\" data-end=\"4756\">Answer<\/strong>: CD34 is the most widely used marker to identify hematopoietic stem cells, although other markers like CD38, Sca-1, and CD133 are often used in combination for more precise identification.<\/p>\n<\/li>\n<\/ul>\n<\/li>\n<li data-start=\"4947\" data-end=\"5163\">\n<p data-start=\"4950\" data-end=\"5002\"><strong data-start=\"4950\" data-end=\"5002\">Are CD34+ cells always hematopoietic stem cells?<\/strong><\/p>\n<ul data-start=\"5006\" data-end=\"5163\">\n<li data-start=\"5006\" data-end=\"5163\">\n<p data-start=\"5008\" data-end=\"5163\"><strong data-start=\"5008\" data-end=\"5018\">Answer<\/strong>: No, not all CD34+ cells are HSCs. CD34 is also expressed on hematopoietic progenitor cells, which are more differentiated than true stem cells.<\/p>\n<\/li>\n<\/ul>\n<\/li>\n<li data-start=\"5165\" data-end=\"5415\">\n<p data-start=\"5168\" data-end=\"5217\"><strong data-start=\"5168\" data-end=\"5217\">What is the role of CD38 in identifying HSCs?<\/strong><\/p>\n<ul data-start=\"5221\" data-end=\"5415\">\n<li data-start=\"5221\" data-end=\"5415\">\n<p data-start=\"5223\" data-end=\"5415\"><strong data-start=\"5223\" data-end=\"5233\">Answer<\/strong>: CD38 is a marker for more differentiated blood cells, and HSCs are typically CD38-. Therefore, a CD34+ CD38- phenotype is commonly used to enrich for true hematopoietic stem cells.<\/p>\n<\/li>\n<\/ul>\n<\/li>\n<li data-start=\"5417\" data-end=\"5659\">\n<p data-start=\"5420\" data-end=\"5465\"><strong data-start=\"5420\" data-end=\"5465\">Can these markers be used for human HSCs?<\/strong><\/p>\n<ul data-start=\"5469\" data-end=\"5659\">\n<li data-start=\"5469\" data-end=\"5659\">\n<p data-start=\"5471\" data-end=\"5659\"><strong data-start=\"5471\" data-end=\"5481\">Answer<\/strong>: Yes, many of these markers, including CD34, CD133, and CD90, are also used to identify human hematopoietic stem cells, though some (like Sca-1) are more common in mouse models.<\/p>\n<\/li>\n<\/ul>\n<\/li>\n<li data-start=\"5661\" data-end=\"5979\" data-is-last-node=\"\">\n<p data-start=\"5664\" data-end=\"5744\"><strong data-start=\"5664\" data-end=\"5744\">What is the significance of Lin- cells in hematopoietic stem cell isolation?<\/strong><\/p>\n<ul data-start=\"5748\" data-end=\"5979\" data-is-last-node=\"\">\n<li data-start=\"5748\" data-end=\"5979\" data-is-last-node=\"\">\n<p data-start=\"5750\" data-end=\"5979\" data-is-last-node=\"\"><strong data-start=\"5750\" data-end=\"5760\">Answer<\/strong>: Lin- cells lack the expression of surface markers associated with mature blood cell types, making them highly enriched for hematopoietic stem cells and progenitors. This phenotype is critical for isolating stem cells.<\/p>\n<\/li>\n<\/ul>\n<\/li>\n<\/ol><\/div>","protected":false},"excerpt":{"rendered":"<p>Hematopoietic stem cells (HSCs) are the progenitors of all blood cell types, including red blood cells, white blood cells, and platelets. These cells are typically found in the bone marrow and are essential for maintaining the body\u2019s blood cell population throughout life. Identifying and isolating HSCs is crucial for research in regenerative medicine and stem&#8230;<\/p>","protected":false},"author":11,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[1],"tags":[],"class_list":["post-12591","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"acf":[],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack-related-posts":[],"_links":{"self":[{"href":"https:\/\/stemlix.com\/en\/wp-json\/wp\/v2\/posts\/12591","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/stemlix.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/stemlix.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/stemlix.com\/en\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/stemlix.com\/en\/wp-json\/wp\/v2\/comments?post=12591"}],"version-history":[{"count":1,"href":"https:\/\/stemlix.com\/en\/wp-json\/wp\/v2\/posts\/12591\/revisions"}],"predecessor-version":[{"id":12832,"href":"https:\/\/stemlix.com\/en\/wp-json\/wp\/v2\/posts\/12591\/revisions\/12832"}],"wp:attachment":[{"href":"https:\/\/stemlix.com\/en\/wp-json\/wp\/v2\/media?parent=12591"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/stemlix.com\/en\/wp-json\/wp\/v2\/categories?post=12591"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/stemlix.com\/en\/wp-json\/wp\/v2\/tags?post=12591"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}